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Wash the sample 3 times with PBS, fix with 4% paraformaldehyde for 15 min, and then treat with 0.5% Triton X-100 for 15 min

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PMC11499218 Hou, A., Cohen, B., Haimovic, A., & Elbuluk, N

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These clusters are reminiscent of other disease clusters stemming from community infectious or toxic exposure